Small Interfering RNA-Induced Transcriptional Gene Silencing in Human Cells
University of California, Los Angeles · University of California San Diego · +1 more institution
Abstract
Small interfering RNA (siRNA) and microRNA silence genes at the transcriptional, posttranscriptional, and/or translational level. Using human tissue culture cells, we show that promoter-directed siRNA inhibits transcription of an integrated, proviral, elongation factor 1alpha (EF1A) promoter-green fluorescent protein reporter gene and of endogenous EF1A. Silencing was associated with DNA methylation of the targeted sequence, and it required either active transport of siRNA into the nucleus or permeabilization of the nuclear envelope by lentiviral transduction. These results demonstrate that siRNA-directed transcriptional silencing is conserved in mammals, providing a means to inhibit mammalian gene function.
Citation impact
- FWCI
- 33.63
- Percentile
- 100%
- References
- 31
Authors
4- KVKevin V. MorrisCorresponding
University of California, Los Angeles, University of California San Diego, VA San Diego Healthcare System
- WCWan Chan
University of California, Los Angeles, University of California San Diego, VA San Diego Healthcare System
- SESteven E. Jacobsen
University of California, Los Angeles, University of California San Diego, VA San Diego Healthcare System
- DJDavid J. Looney
University of California, Los Angeles, University of California San Diego, VA San Diego Healthcare System
Topics & keywords
- Gene silencing
- Small interfering RNA
- Trans-acting siRNA
- Biology
- RNA-induced silencing complex
- RNA silencing
- Cell biology
- RNA interference