Widespread Translational Inhibition by Plant miRNAs and siRNAs
Centre National de la Recherche Scientifique · University of Utah · +2 more institutions
Abstract
High complementarity between plant microRNAs (miRNAs) and their messenger RNA targets is thought to cause silencing, prevalently by endonucleolytic cleavage. We have isolated Arabidopsis mutants defective in miRNA action. Their analysis provides evidence that plant miRNA-guided silencing has a widespread translational inhibitory component that is genetically separable from endonucleolytic cleavage. We further show that the same is true of silencing mediated by small interfering RNA (siRNA) populations. Translational repression is effected in part by the ARGONAUTE proteins AGO1 and AGO10. It also requires the activity of the microtubule-severing enzyme katanin, implicating cytoskeleton dynamics in miRNA action,…
Citation impact
- FWCI
- 156.10
- Percentile
- 100%
- References
- 45
Authors
7- PBPeter BrodersenCorresponding
Centre National de la Recherche Scientifique, University of Utah, Institut de Biologie Moléculaire des Plantes, Nagoya University
- LSLali Sakvarelidze-Achard
Centre National de la Recherche Scientifique, University of Utah, Institut de Biologie Moléculaire des Plantes, Nagoya University
- MBM. Bruun-Rasmussen
Centre National de la Recherche Scientifique, University of Utah, Institut de Biologie Moléculaire des Plantes, Nagoya University
- PDPatrice Dunoyer
Centre National de la Recherche Scientifique, University of Utah, Institut de Biologie Moléculaire des Plantes, Nagoya University
- YYYoshiharu Y. Yamamoto
Centre National de la Recherche Scientifique, University of Utah, Institut de Biologie Moléculaire des Plantes, Nagoya University
Topics & keywords
- Argonaute
- Gene silencing
- Biology
- microRNA
- Small interfering RNA
- Trans-acting siRNA
- Cell biology
- RNA silencing