What Macromolecular Crowding Can Do to a Protein
Peter the Great St. Petersburg Polytechnic University · Institute of Cytology · +4 more institutions
Abstract
The intracellular environment represents an extremely crowded milieu, with a limited amount of free water and an almost complete lack of unoccupied space. Obviously, slightly salted aqueous solutions containing low concentrations of a biomolecule of interest are too simplistic to mimic the "real life" situation, where the biomolecule of interest scrambles and wades through the tightly packed crowd. In laboratory practice, such macromolecular crowding is typically mimicked by concentrated solutions of various polymers that serve as model "crowding agents". Studies under these conditions revealed that macromolecular crowding might affect protein structure, folding, shape, conformational stability, binding of…
Citation impact
- FWCI
- 13.51
- Percentile
- 100%
- References
- 331
Authors
3- IМIrina М. Kuznetsova
Peter the Great St. Petersburg Polytechnic University, Institute of Cytology
- KКKonstantin К. Turoverov
Peter the Great St. Petersburg Polytechnic University, Institute of Cytology
- VNVladimir N. UverskyCorresponding
King Abdulaziz University, University of South Florida, Institute of Cytology, Institute for Biological Instrumentation, USF Health Byrd Alzheimer's Institute
Topics & keywords
- Macromolecular crowding
- Biomolecule
- Macromolecule
- Crowding
- Folding (DSP implementation)
- Protein folding
- Macromolecular Substances
- Nucleic acid
- Clean water and sanitation