Human colorectal cancer-specific CCAT1-L lncRNA regulates long-range chromatin interactions at the MYC locus
Center for Excellence in Molecular Cell Science · Chinese Academy of Sciences · +2 more institutions
Abstract
The human 8q24 gene desert contains multiple enhancers that form tissue-specific long-range chromatin loops with the MYC oncogene, but how chromatin looping at the MYC locus is regulated remains poorly understood. Here we demonstrate that a long noncoding RNA (lncRNA), CCAT1-L, is transcribed specifically in human colorectal cancers from a locus 515 kb upstream of MYC. This lncRNA plays a role in MYC transcriptional regulation and promotes long-range chromatin looping. Importantly, the CCAT1-L locus is located within a strong super-enhancer and is spatially close to MYC. Knockdown of CCAT1-L reduced long-range interactions between the MYC promoter and its enhancers. In addition, CCAT1-L interacts with CTCF and…
Citation impact
- FWCI
- 21.68
- Percentile
- 100%
- References
- 77
Authors
12- JXJian‐Feng XiangCorresponding
Center for Excellence in Molecular Cell Science
- QYQing-Fei Yin
Center for Excellence in Molecular Cell Science
- TCTian Chen
Center for Excellence in Molecular Cell Science
- YZYang Zhang
Center for Excellence in Molecular Cell Science
- XZXiao‐Ou Zhang
Chinese Academy of Sciences, Shanghai Institutes for Biological Sciences
Topics & keywords
- Chromatin
- Biology
- Enhancer
- Locus (genetics)
- CTCF
- Long non-coding RNA
- Gene knockdown
- Chromatin remodeling