A Genetically Encoded Tag for Correlated Light and Electron Microscopy of Intact Cells, Tissues, and Organisms
Howard Hughes Medical Institute · University of California San Diego · +2 more institutions
Abstract
Electron microscopy (EM) achieves the highest spatial resolution in protein localization, but specific protein EM labeling has lacked generally applicable genetically encoded tags for in situ visualization in cells and tissues. Here we introduce "miniSOG" (for mini Singlet Oxygen Generator), a fluorescent flavoprotein engineered from Arabidopsis phototropin 2. MiniSOG contains 106 amino acids, less than half the size of Green Fluorescent Protein. Illumination of miniSOG generates sufficient singlet oxygen to locally catalyze the polymerization of diaminobenzidine into an osmiophilic reaction product resolvable by EM. MiniSOG fusions to many well-characterized proteins localize correctly in mammalian cells,…
Citation impact
- FWCI
- 80.36
- Percentile
- 100%
- References
- 57
Authors
9- XSXiaokun ShuCorresponding
Howard Hughes Medical Institute, University of California San Diego
- VLVarda Lev‐Ram
University of California San Diego
- TJThomas J. Deerinck
University of California San Diego
- YQYingchuan Qi
Howard Hughes Medical Institute, University of California San Diego
- EBEricka B. Ramko
Florida State University, National High Magnetic Field Laboratory
Topics & keywords
- Biology
- Yellow fluorescent protein
- Fluorescence
- Electron microscope
- Protein tag
- Fluorescence microscope
- Microscopy
- Biophysics