Mass Spectrometry-Based Label-Free Quantitative Proteomics
Sanford Burnham Prebys Medical Discovery Institute · Pathways Behavioral Services · +1 more institution
Abstract
In order to study the differential protein expression in complex biological samples, strategies for rapid, highly reproducible and accurate quantification are necessary. Isotope labeling and fluorescent labeling techniques have been widely used in quantitative proteomics research. However, researchers are increasingly turning to label-free shotgun proteomics techniques for faster, cleaner, and simpler results. Mass spectrometry-based label-free quantitative proteomics falls into two general categories. In the first are the measurements of changes in chromatographic ion intensity such as peptide peak areas or peak heights. The second is based on the spectral counting of identified proteins. In this paper, we…
Citation impact
- FWCI
- 19.52
- Percentile
- 100%
- References
- 43
Authors
3- WZWenhong ZhuCorresponding
Sanford Burnham Prebys Medical Discovery Institute, Pathways Behavioral Services
- JWJeffrey W. Smith
Sanford Burnham Prebys Medical Discovery Institute, University of California San Diego, Pathways Behavioral Services
- CHChun‐Ming HuangCorresponding
University of California San Diego
Topics & keywords
- Quantitative proteomics
- Label-free quantification
- Proteomics
- Shotgun proteomics
- Mass spectrometry
- Stable isotope labeling by amino acids in cell culture
- Computer science
- Chemistry