PERIOD2::LUCIFERASE real-time reporting of circadian dynamics reveals persistent circadian oscillations in mouse peripheral tissues
Northwestern University · U.S. National Science Foundation · +5 more institutions
Abstract
Mammalian circadian rhythms are regulated by the suprachiasmatic nucleus (SCN), and current dogma holds that the SCN is required for the expression of circadian rhythms in peripheral tissues. Using a PERIOD2::LUCIFERASE fusion protein as a real-time reporter of circadian dynamics in mice, we report that, contrary to previous work, peripheral tissues are capable of self-sustained circadian oscillations for >20 cycles in isolation. In addition, peripheral organs expressed tissue-specific differences in circadian period and phase. Surprisingly, lesions of the SCN in mPer2(Luciferase) knockin mice did not abolish circadian rhythms in peripheral tissues, but instead caused phase desynchrony among the tissues of…
Citation impact
- FWCI
- 24.72
- Percentile
- 100%
- References
- 41
Authors
12- SHSeung Hee YooCorresponding
Northwestern University, U.S. National Science Foundation, Howard Hughes Medical Institute, Korea Advanced Institute of Science and Technology, University of Toronto, Foundation Center
- SYShin Yamazaki
Northwestern University, U.S. National Science Foundation, Howard Hughes Medical Institute, Korea Advanced Institute of Science and Technology, University of Toronto, Vanderbilt University, Foundation Center
- PLPhillip L. Lowrey
Northwestern University, U.S. National Science Foundation, Howard Hughes Medical Institute, Korea Advanced Institute of Science and Technology, University of Toronto, Foundation Center
- KSKazuhiro Shimomura
Northwestern University, U.S. National Science Foundation, Howard Hughes Medical Institute, Korea Advanced Institute of Science and Technology, University of Toronto, Foundation Center
- CHCaroline H. Ko
Northwestern University, U.S. National Science Foundation, Howard Hughes Medical Institute, Korea Advanced Institute of Science and Technology, University of Toronto, Foundation Center
Topics & keywords
- Circadian rhythm
- Suprachiasmatic nucleus
- Light effects on circadian rhythm
- Biology
- Bacterial circadian rhythms
- Period (music)
- Peripheral
- Endocrinology