Exosome Uptake through Clathrin-mediated Endocytosis and Macropinocytosis and Mediating miR-21 Delivery
State Key Laboratory of Digital Medical Engineering · Southeast University · +1 more institution
Abstract
Exosomes are nanoscale membrane vesicles secreted from many types of cells. Carrying functional molecules, exosomes transfer information between cells and mediate many physiological and pathological processes. In this report, utilizing selective inhibitors, molecular tools, and specific endocytosis markers, the cellular uptake of PC12 cell-derived exosomes was imaged by high-throughput microscopy and statistically analyzed. It was found that the uptake was through clathrin-mediated endocytosis and macropinocytosis. Furthermore, PC12 cell-derived exosomes can enter and deliver microRNAs (miRNAs) into bone marrow-derived mesenchymal stromal cells (BMSCs), and decrease the expression level of transforming growth…
Citation impact
- FWCI
- 13.52
- Percentile
- 100%
- References
- 42
Authors
7- TTTian Tian
State Key Laboratory of Digital Medical Engineering, Southeast University, Nanjing Medical University
- YZYan-Liang Zhu
State Key Laboratory of Digital Medical Engineering, Southeast University
- YZYue-Yuan Zhou
Southeast University, State Key Laboratory of Digital Medical Engineering
- GLGao-Feng Liang
Southeast University, State Key Laboratory of Digital Medical Engineering
- YWYuan-Yuan Wang
Southeast University, State Key Laboratory of Digital Medical Engineering
Topics & keywords
- Endocytosis
- Microvesicles
- Pinocytosis
- Internalization
- Cell biology
- Exosome
- Clathrin
- Biology
Funding
- NNNational Natural Science Foundation of ChinaAwards: 31371003, 61071047
- GOGovernment of Jiangsu Province
- NSNatural Science Foundation of Jiangsu ProvinceAwards: BK2012122, BK20130886, No. BK2012122
- NKNational Key Research and Development Program of ChinaAwards: 973 Program: 2013CB932902, 2013CB932902
- SRSpecialized Research Fund for the Doctoral Program of Higher Education of ChinaAward: 20130092110030