articleProceedings of the National Academy of SciencesMar 21, 2007GREEN OA

Distribution of hematopoietic stem cells in the bone marrow according to regional hypoxia

KPKalindi ParmarPMPeter MauchJVJo‐Anne VergilioRSRobert SacksteinJDJulian D. Down

Dana-Farber Cancer Institute · Brigham and Women's Hospital · +1 more institution

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Abstract

The interaction of stem cells with their bone marrow microenvironment is a critical process in maintaining normal hematopoiesis. We applied an approach to resolve the spatial organization that underlies these interactions by evaluating the distribution of hematopoietic cell subsets along an in vivo Hoechst 33342 (Ho) dye perfusion gradient. Cells isolated from different bone marrow regions according to Ho fluorescence intensity contained the highest concentration of hematopoietic stem cell (HSC) activity in the lowest end of the Ho gradient (i.e., in the regions reflecting diminished perfusion). Consistent with the ability of Ho perfusion to simulate the level of oxygenation, bone marrow fractions separately…

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Topics & keywords

Topics
Keywords
  • Stem cell
  • Bone marrow
  • Haematopoiesis
  • Hematopoietic stem cell
  • Hypoxia (environmental)
  • Perfusion
  • Biology
  • Cell biology
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