WIPI2 Links LC3 Conjugation with PI3P, Autophagosome Formation, and Pathogen Clearance by Recruiting Atg12–5-16L1
The Honourable Society of Lincoln's Inn · Cancer Research UK · +2 more institutions
Abstract
Mammalian cell homeostasis during starvation depends on initiation of autophagy by endoplasmic reticulum-localized phosphatidylinositol 3-phosphate (PtdIns(3)P) synthesis. Formation of double-membrane autophagosomes that engulf cytosolic components requires the LC3-conjugating Atg12-5-16L1 complex. The molecular mechanisms of Atg12-5-16L1 recruitment and significance of PtdIns(3)P synthesis at autophagosome formation sites are unknown. By identifying interacting partners of WIPIs, WD-repeat PtdIns(3)P effector proteins, we found that Atg16L1 directly binds WIPI2b. Mutation experiments and ectopic localization of WIPI2b to plasma membrane show that WIPI2b is a PtdIns(3)P effector upstream of Atg16L1 and is…
Citation impact
- FWCI
- 33.64
- Percentile
- 100%
- References
- 46
Authors
6- HCHannah C. DooleyCorresponding
The Honourable Society of Lincoln's Inn, Cancer Research UK
- MRMinoo Razi
The Honourable Society of Lincoln's Inn, Cancer Research UK
- HEHannah E.J. Polson
The Honourable Society of Lincoln's Inn, Cancer Research UK
- SEStephen E. Girardin
University of Toronto
- MWMichael Wilson
Babraham Institute
Topics & keywords
- Autophagy
- ATG16L1
- Cell biology
- ATG12
- Autophagosome
- Biology
- Effector
- Phosphatidylinositol