Single‐step isolation of extracellular vesicles by size‐exclusion chromatography

Isolation of extracellular vesicles from plasma is a challenge due to the presence of proteins and lipoproteins. Isolation of vesicles using differential centrifugation or density-gradient ultracentrifugation results in co-isolation of contaminants such as protein aggregates and incomplete separation of vesicles from lipoproteins, respectively.

To develop a single-step protocol to isolate vesicles from human body fluids.