Endosomal Proteolysis of the Ebola Virus Glycoprotein Is Necessary for Infection
Brigham and Women's Hospital · National Institutes of Health · +4 more institutions
Abstract
Ebola virus (EboV) causes rapidly fatal hemorrhagic fever in humans and there is currently no effective treatment. We found that the infection of African green monkey kidney (Vero) cells by vesicular stomatitis viruses bearing the EboV glycoprotein (GP) requires the activity of endosomal cysteine proteases. Using selective protease inhibitors and protease-deficient cell lines, we identified an essential role for cathepsin B (CatB) and an accessory role for cathepsin L (CatL) in EboV GP-dependent entry. Biochemical studies demonstrate that CatB and CatL mediate entry by carrying out proteolysis of the EboV GP subunit GP1 and support a multistep mechanism that explains the relative contributions of these enzymes…
Citation impact
- FWCI
- 16.48
- Percentile
- 100%
- References
- 24
Authors
5- KCKartik Chandran
Brigham and Women's Hospital, National Institutes of Health, Harvard University, University of Würzburg, Institute of Human Genetics, National Institute of Allergy and Infectious Diseases
- NJNancy J. Sullivan
Brigham and Women's Hospital, National Institutes of Health, Harvard University, University of Würzburg, Institute of Human Genetics, National Institute of Allergy and Infectious Diseases
- UFUte Felbor
Brigham and Women's Hospital, National Institutes of Health, Harvard University, University of Würzburg, Institute of Human Genetics, National Institute of Allergy and Infectious Diseases
- SPSean P. J. Whelan
Brigham and Women's Hospital, National Institutes of Health, Harvard University, University of Würzburg, Institute of Human Genetics, National Institute of Allergy and Infectious Diseases
- JMJames M. CunninghamCorresponding
Brigham and Women's Hospital, National Institutes of Health, Harvard University, University of Würzburg, Institute of Human Genetics, National Institute of Allergy and Infectious Diseases
Topics & keywords
- Ebola virus
- Filoviridae
- Virology
- Cathepsin L
- Vero cell
- Biology
- Cathepsin B
- Proteases
- Good health and well-being