High Cleavage Efficiency of a 2A Peptide Derived from Porcine Teschovirus-1 in Human Cell Lines, Zebrafish and Mice
Institute of Medical Sciences · Chonnam National University Hospital · +7 more institutions
Abstract
When expression of more than one gene is required in cells, bicistronic or multicistronic expression vectors have been used. Among various strategies employed to construct bicistronic or multicistronic vectors, an internal ribosomal entry site (IRES) has been widely used. Due to the large size and difference in expression levels between genes before and after IRES, however, a new strategy was required to replace IRES. A self-cleaving 2A peptide could be a good candidate to replace IRES because of its small size and high cleavage efficiency between genes upstream and downstream of the 2A peptide. Despite the advantages of the 2A peptides, its use is not widespread because (i) there are no publicly available…
Citation impact
- FWCI
- 16.24
- Percentile
- 100%
- References
- 18
Authors
9- JHJin Hee Kim
Institute of Medical Sciences, Chonnam National University Hospital, Chonnam National University
- SRSang Rok Lee
Junta de Andalucía, Gwangju University, Chosun University, Chonnam National University Hwasun Hospital
- LLLi-Hua Li
Institute of Medical Sciences, Chonnam National University Hospital, Hainan University, Hainan Medical University, Chonnam National University
- HPHye‐Jeong Park
Gwangju University, Chonnam National University, Chosun University, Chonnam National University Hospital
- JPJeong-Hoh Park
Chonnam National University
Topics & keywords
- Biology
- Cleavage (geology)
- Internal ribosome entry site
- Gene
- Molecular biology
- Expression cloning
- Cloning (programming)
- Plasmid