articleBloodMay 27, 2005BRONZE OA

Development of functional human blood and immune systems in NOD/SCID/IL2 receptor γ chainnull mice

FIFumihiko IshikawaMYMasaki YasukawaBLBonnie LyonsSYShuro YoshidaTMToshihiro Miyamoto

Kyushu University · Kyushu University Hospital · +4 more institutions

PubMed
Indexed incrossrefpubmed

Abstract

Here we report that a new nonobese diabetic/severe combined immunodeficient (NOD/SCID) mouse line harboring a complete null mutation of the common cytokine receptor gamma chain (NOD/SCID/interleukin 2 receptor [IL2r] gamma(null)) efficiently supports development of functional human hemato-lymphopoiesis. Purified human (h) CD34(+) or hCD34(+)hCD38(-) cord blood (CB) cells were transplanted into NOD/SCID/IL2rgamma(null) newborns via a facial vein. In all recipients injected with 10(5) hCD34(+) or 2 x 10(4) hCD34(+)hCD38(-) CB cells, human hematopoietic cells were reconstituted at approximately 70% of chimerisms. A high percentage of the human hematopoietic cell chimerism persisted for more than 24 weeks after…

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Topics & keywords

Topics
Keywords
  • Biology
  • Haematopoiesis
  • Nod
  • Immunology
  • Stem cell
  • Lymphopoiesis
  • Myeloid
  • Severe combined immunodeficiency
UN Sustainable Development Goals
  • Good health and well-being
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