Versatile, Fully Automated, Microfluidic Cell Culture System
Howard Hughes Medical Institute · Stanford University · +1 more institution
Abstract
There is increasing demand for automated and quantitative cell culture technology, driven both by the intense activity in stem cell biology and by the emergence of systems biology. We built a fully automated cell culture screening system based on a microfluidic chip that creates arbitrary culture media formulations in 96 independent culture chambers and maintains cell viability for weeks. Individual culture conditions are customized in terms of cell seeding density, composition of culture medium, and feeding schedule, and each chamber is imaged with time-lapse microscopy. Using this device, we perform the first quantitative measurements of the influence of transient stimulation schedules on the proliferation,…
Citation impact
- FWCI
- 34.49
- Percentile
- 100%
- References
- 19
Authors
5- RGRafael Gómez-SjöbergCorresponding
Howard Hughes Medical Institute, Stanford University, University of Pennsylvania
- ALAnne Leyrat
Stanford University, University of Pennsylvania, Howard Hughes Medical Institute
- DMDana M. Pirone
University of Pennsylvania, Howard Hughes Medical Institute, Stanford University
- CSChristopher S. Chen
University of Pennsylvania, Howard Hughes Medical Institute, Stanford University
- SRStephen R. Quake
Howard Hughes Medical Institute, University of Pennsylvania, Stanford University
Topics & keywords
- Microfluidics
- Cell culture
- Chemistry
- Mesenchymal stem cell
- Cell biology
- Motility
- Cell
- Seeding