Transcellular degradation of axonal mitochondria
Kennedy Krieger Institute · Johns Hopkins University · +2 more institutions
Abstract
It is generally accepted that healthy cells degrade their own mitochondria. Here, we report that retinal ganglion cell axons of WT mice shed mitochondria at the optic nerve head (ONH), and that these mitochondria are internalized and degraded by adjacent astrocytes. EM demonstrates that mitochondria are shed through formation of large protrusions that originate from otherwise healthy axons. A virally introduced tandem fluorophore protein reporter of acidified mitochondria reveals that acidified axonal mitochondria originating from the retinal ganglion cell are associated with lysosomes within columns of astrocytes in the ONH. According to this reporter, a greater proportion of retinal ganglion cell…
Citation impact
- FWCI
- 16.79
- Percentile
- 100%
- References
- 29
Authors
14- COChung-ha O. DavisCorresponding
Kennedy Krieger Institute, Johns Hopkins University, Johns Hopkins Medicine
- KKKeun-Young Kim
University of California San Diego
- EAEric A. Bushong
University of California San Diego
- EMElizabeth Mills
Kennedy Krieger Institute, Johns Hopkins University, Johns Hopkins Medicine
- DBDaniela Boassa
University of California San Diego
Topics & keywords
- Transcellular
- Degradation (telecommunications)
- Mitochondrion
- Neuroscience
- Cell biology
- Chemistry
- Biophysics
- Biology