MicroRNA-126 regulates endothelial expression of vascular cell adhesion molecule 1
Johns Hopkins University · Johns Hopkins Medicine · +2 more institutions
Abstract
Adhesion molecules expressed by activated endothelial cells play a key role in regulating leukocyte trafficking to sites of inflammation. Resting endothelial cells normally do not express adhesion molecules, but cytokines activate endothelial cells to express adhesion molecules such as vascular cell adhesion molecule 1 (VCAM-1), which mediate leukocyte adherence to endothelial cells. We now show that endothelial cells express microRNA 126 (miR-126), which inhibits VCAM-1 expression. Transfection of endothelial cells with an oligonucleotide that decreases miR-126 permits an increase in TNF-alpha-stimulated VCAM-1 expression. Conversely, overexpression of the precursor to miR-126 increases miR-126 levels and…
Citation impact
- FWCI
- 18.36
- Percentile
- 100%
- References
- 22
Authors
5- THTamia Harris-TryonCorresponding
Johns Hopkins University, Johns Hopkins Medicine
- MYMunekazu Yamakuchi
Johns Hopkins University
- MFMarcella Ferlito
Johns Hopkins University
- JTJoshua T. Mendell
Johns Hopkins University, Johns Hopkins Medicine, Institut thématique Génétique, génomique et bioinformatique, Pediatrics and Genetics
- CJCharles J. Lowenstein
Johns Hopkins University
Topics & keywords
- Cell adhesion molecule
- Cell biology
- Cell adhesion
- Transfection
- VCAM-1
- Endothelial stem cell
- microRNA
- Soluble cell adhesion molecules