articleProceedings of the National Academy of SciencesJun 7, 2010GREEN OA

Escherichia coli induces DNA damage in vivo and triggers genomic instability in mammalian cells

GCGabriel Cuevas‐RamosCPC. PetitIMIngrid MarcqMBMichèle BouryÉOÉric Oswald

École Nationale Vétérinaire de Toulouse · Université Fédérale de Toulouse Midi-Pyrénées · +3 more institutions

PubMed
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Abstract

Escherichia coli is a normal inhabitant of the human gut. However, E. coli strains of phylogenetic group B2 harbor a genomic island called "pks" that codes for the production of a polyketide-peptide genotoxin, Colibactin. Here we report that in vivo infection with E. coli harboring the pks island, but not with a pks isogenic mutant, induced the formation of phosphorylated H2AX foci in mouse enterocytes. We show that a single, short exposure of cultured mammalian epithelial cells to live pks(+) E. coli at low infectious doses induced a transient DNA damage response followed by cell division with signs of incomplete DNA repair, leading to anaphase bridges and chromosome aberrations. Micronuclei, aneuploidy, ring…

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849
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Authors

6

Topics & keywords

Topics
Keywords
  • Biology
  • Genome instability
  • DNA damage
  • Escherichia coli
  • Chromosome instability
  • Anaphase
  • Mutant
  • DNA repair
UN Sustainable Development Goals
  • Life below water
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