Rolling circle amplification: a versatile tool for chemical biology, materials science and medicine
University of California, Irvine · University of Alberta · +4 more institutions
Abstract
Rolling circle amplification (RCA) is an isothermal enzymatic process where a short DNA or RNA primer is amplified to form a long single stranded DNA or RNA using a circular DNA template and special DNA or RNA polymerases. The RCA product is a concatemer containing tens to hundreds of tandem repeats that are complementary to the circular template. The power, simplicity, and versatility of the DNA amplification technique have made it an attractive tool for biomedical research and nanobiotechnology. Traditionally, RCA has been used to develop sensitive diagnostic methods for a variety of targets including nucleic acids (DNA, RNA), small molecules, proteins, and cells. RCA has also attracted significant attention…
Citation impact
- FWCI
- 24.47
- Percentile
- 100%
- References
- 166
Authors
8- MMM. Monsur Ali
University of California, Irvine
- FLFeng Li
University of Alberta, University of California, Irvine, Edwards Lifesciences (United States)
- ZZZhiqing Zhang
China University of Petroleum, East China, Edwards Lifesciences (United States), University of California, Irvine
- KZKaixiang Zhang
University of California, Irvine, Tsinghua University, Edwards Lifesciences (United States)
- DKDong‐Ku Kang
University of California, Irvine, Edwards Lifesciences (United States)
Topics & keywords
- Rolling circle replication
- Nanobiotechnology
- Nanotechnology
- DNA
- DNA nanotechnology
- DNA origami
- RNA
- Nucleic acid