Direct recognition of the mycobacterial glycolipid, trehalose dimycolate, by C-type lectin Mincle
Kyushu University · Center of Molecular Immunology (Cuba) · +4 more institutions
Abstract
Tuberculosis remains a fatal disease caused by Mycobacterium tuberculosis, which contains various unique components that affect the host immune system. Trehalose-6,6'-dimycolate (TDM; also called cord factor) is a mycobacterial cell wall glycolipid that is the most studied immunostimulatory component of M. tuberculosis. Despite five decades of research on TDM, its host receptor has not been clearly identified. Here, we demonstrate that macrophage inducible C-type lectin (Mincle) is an essential receptor for TDM. Heat-killed mycobacteria activated Mincle-expressing cells, but the activity was lost upon delipidation of the bacteria; analysis of the lipid extracts identified TDM as a Mincle ligand. TDM activated…
Citation impact
- FWCI
- 15.61
- Percentile
- 100%
- References
- 48
Authors
10- EIEri IshikawaCorresponding
Kyushu University, Center of Molecular Immunology (Cuba)
- TITetsuaki Ishikawa
Kyushu University, Chiba University, Center of Molecular Immunology (Cuba)
- YSYasu S. Morita
Laboratory of Molecular Genetics, Microbial Chemistry Research Foundation, The University of Osaka
- KTKenji Toyonaga
Kyushu University, Center of Molecular Immunology (Cuba)
- HYHisakata Yamada
Kyushu University, Center of Molecular Immunology (Cuba)
Topics & keywords
- Glycolipid
- C-type lectin
- Macrophage
- Microbiology
- Mycobacterium tuberculosis
- Biology
- Immune system
- Lectin
- Good health and well-being