Activation of the PTEN/mTOR/STAT3 pathway in breast cancer stem-like cells is required for viability and maintenance

Side-population (SP) cells within cancers and cell lines are rare cell populations known to enrich cancer stem-like cells. In this study, we characterized SP cells from the human breast cancer cell line MCF7 as a model for cancer stem-like cells. Compared with non-SP cells, MCF7 SP cells had higher colony-formation ability in vitro and greater tumorigenicity in vivo, suggesting that MCF7 SP cells enrich cancer stem-like cells. cDNA microarray analysis of the SP cells indicated higher expression of ATP-binding cassette transporters and genes involved in quiescence, which were confirmed by quantitative RT-PCR and flow cytometry cell cycle analysis. To identify signal pathways important for cancer stem-like…

• GM
  George Mason University
• JH
  Johns Hopkins University
• NI
  National Institutes of Health
• CF
  Center for AIDS Research, University of Washington
• JH
  Johns Hopkins Bloomberg School of Public Health
• CO
  College of Science, George Mason University
• CF
  Center for AIDS Research, Johns Hopkins University