Mapping of picoeucaryotes in marine ecosystems with quantitative PCR of the 18S rRNA gene
Centre National de la Recherche Scientifique · Station Biologique de Roscoff · +3 more institutions
Abstract
A quantitative PCR (QPCR) assay based on the use of SYBR Green I was developed to assess the abundance of specific groups of picoeukaryotes in marine waters. Six primer sets were designed targeting four different taxonomic levels: domain (Eukaryota), division (Chlorophyta), order (Mamiellales) and genus (Bathycoccus, Micromonas, and Ostreococcus). Reaction conditions were optimized for each primer set which was validated in silico, on agarose gels, and by QPCR against a variety of target and non-target cultures. The approach was tested by estimating gene copy numbers for Micromonas, Bathycoccus, and Ostreococcus in seawater samples to which cultured cells were added in various concentrations. QPCR was then…
Citation impact
- FWCI
- 6.97
- Percentile
- 100%
- References
- 56
Authors
5- FZFei ZhuCorresponding
Centre National de la Recherche Scientifique, Station Biologique de Roscoff
- RMRamón Massana
Institut de Ciències del Mar, Institut Català de Ciències del Clima, Centro Mediterráneo de Investigaciones Marinas y Ambientale
- FNFabrice Not
Station Biologique de Roscoff, Centre National de la Recherche Scientifique
- DMDominique Marie
Station Biologique de Roscoff, Centre National de la Recherche Scientifique
- DVDaniel Vaulot
Station Biologique de Roscoff, Centre National de la Recherche Scientifique
Topics & keywords
- Biology
- Chlorophyta
- Primer (cosmetics)
- Picoplankton
- Phytoplankton
- Botany
- Ecology
- Algae
- Life below water