articlePLoS GeneticsJan 5, 2012GOLD OA

Contribution of Intragenic DNA Methylation in Mouse Gametic DNA Methylomes to Establish Oocyte-Specific Heritable Marks

HKHisato KobayashiTSTakayuki SakuraiMIMisaki ImaiNTNozomi TakahashiAFAtsushi Fukuda

Tokyo University of Agriculture · National Center For Child Health and Development · +2 more institutions

PubMed
Indexed incrossrefdoajpubmed

Abstract

Genome-wide dynamic changes in DNA methylation are indispensable for germline development and genomic imprinting in mammals. Here, we report single-base resolution DNA methylome and transcriptome maps of mouse germ cells, generated using whole-genome shotgun bisulfite sequencing and cDNA sequencing (mRNA-seq). Oocyte genomes showed a significant positive correlation between mRNA transcript levels and methylation of the transcribed region. Sperm genomes had nearly complete coverage of methylation, except in the CpG-rich regions, and showed a significant negative correlation between gene expression and promoter methylation. Thus, these methylome maps revealed that oocytes and sperms are widely different in the…

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Topics & keywords

Topics
Keywords
  • Biology
  • DNA methylation
  • Genomic imprinting
  • RNA-Directed DNA Methylation
  • Genetics
  • Illumina Methylation Assay
  • Methylation
  • Differentially methylated regions
UN Sustainable Development Goals
  • Life below water
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