Ultra-sensitive liquid biopsy of circulating extracellular vesicles using ExoScreen

Cancer cells secrete small membranous extracellular vesicles (EVs) into their microenvironment and circulation. Although their potential as cancer biomarkers has been promising, the identification and quantification of EVs in clinical samples remains challenging. Here we describe a sensitive and rapid analytical technique for profiling circulating EVs directly from blood samples of patients with colorectal cancer. EVs are captured by two types of antibodies and are detected by photosensitizer-beads, which enables us to detect cancer-derived EVs without a purification step. We also show that circulating EVs can be used for detection of colorectal cancer using the antigen CD147, which is embedded in…

• MO
  Ministry of Education, Culture, Sports, Science and Technology

  Award: P10003
• NI
  National Institute of Biomedical Innovation
• CF
  Council for Science and Technology Policy
• JS
  Japan Society for the Promotion of Science