The Standard European Vector Architecture (SEVA): a coherent platform for the analysis and deployment of complex prokaryotic phenotypes
RSRafael Silva‐RochaEMEsteban Martínez‐GarcíaBCBelén CallesMCMax ChavarríaAAAlejandro Arce‐Rodríguez
Centro Nacional de Biotecnología
Indexed incrossrefdoajpubmed
Abstract
The 'Standard European Vector Architecture' database (SEVA-DB, http://seva.cnb.csic.es) was conceived as a user-friendly, web-based resource and a material clone repository to assist in the choice of optimal plasmid vectors for de-constructing and re-constructing complex prokaryotic phenotypes. The SEVA-DB adopts simple design concepts that facilitate the swapping of functional modules and the extension of genome engineering options to microorganisms beyond typical laboratory strains. Under the SEVA standard, every DNA portion of the plasmid vectors is minimized, edited for flaws in their sequence and/or functionality, and endowed with physical connectivity through three inter-segment insulators that are…
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12Topics & keywords
Topics
Keywords
- Biology
- Plasmid
- Computational biology
- Vector (molecular biology)
- Architecture
- Genome
- Computer science
- Genetics
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