The primary mechanism of attenuation of bacillus Calmette–Guérin is a loss of secreted lytic function required for invasion of lung interstitial tissue
Albert Einstein College of Medicine · Center for Biologics Evaluation and Research · +2 more institutions
Abstract
Tuberculosis remains a leading cause of death worldwide, despite the availability of effective chemotherapy and a vaccine. Bacillus Calmette-Guérin (BCG), the tuberculosis vaccine, is an attenuated mutant of Mycobacterium bovis that was isolated after serial subcultures, yet the functional basis for this attenuation has never been elucidated. A single region (RD1), which is absent in all BCG substrains, was deleted from virulent M. bovis and Mycobacterium tuberculosis strains, and the resulting DeltaRD1 mutants were significantly attenuated for virulence in both immunocompromised and immunocompetent mice. The M. tuberculosis DeltaRD1 mutants were also shown to protect mice against aerosol challenge, in a…
Citation impact
- FWCI
- 13.71
- Percentile
- 100%
- References
- 28
Authors
17- THTsungda HsuCorresponding
Albert Einstein College of Medicine, Center for Biologics Evaluation and Research, Howard Hughes Medical Institute, University of California, Los Angeles
- SHSuzie Hingley‐Wilson
Albert Einstein College of Medicine, Center for Biologics Evaluation and Research, Howard Hughes Medical Institute, University of California, Los Angeles
- BCBing Chen
Albert Einstein College of Medicine, Center for Biologics Evaluation and Research, Howard Hughes Medical Institute, University of California, Los Angeles
- MCMei Chen
Albert Einstein College of Medicine, Center for Biologics Evaluation and Research, Howard Hughes Medical Institute, University of California, Los Angeles
- AZAnnie Z. Dai
Albert Einstein College of Medicine, Center for Biologics Evaluation and Research, Howard Hughes Medical Institute, University of California, Los Angeles
Topics & keywords
- Virulence
- ESAT-6
- Mycobacterium bovis
- Mycobacterium tuberculosis
- Biology
- Microbiology
- Cytolysis
- Mutant
- Good health and well-being