Mass Cytometry: Technique for Real Time Single Cell Multitarget Immunoassay Based on Inductively Coupled Plasma Time-of-Flight Mass Spectrometry
University of Toronto · University Health Network
Abstract
A novel instrument for real time analysis of individual biological cells or other microparticles is described. The instrument is based on inductively coupled plasma time-of-flight mass spectrometry and comprises a three-aperture plasma-vacuum interface, a dc quadrupole turning optics for decoupling ions from neutral components, an rf quadrupole ion guide discriminating against low-mass dominant plasma ions, a point-to-parallel focusing dc quadrupole doublet, an orthogonal acceleration reflectron analyzer, a discrete dynode fast ion detector, and an 8-bit 1 GHz digitizer. A high spectrum generation frequency of 76.8 kHz provides capability for collecting multiple spectra from each particle-induced transient ion…
Citation impact
- FWCI
- 12.59
- Percentile
- 100%
- References
- 30
Authors
10- DBDmitry BanduraCorresponding
University of Toronto, University Health Network
- VBVladimir Baranov
University Health Network, University of Toronto
- OOOlga Ornatsky
University Health Network, University of Toronto
- AAAlexei Antonov
University of Toronto, University Health Network
- RKRobert Kinach
University Health Network, University of Toronto
Topics & keywords
- Chemistry
- Mass spectrometry
- Analytical Chemistry (journal)
- Inductively coupled plasma mass spectrometry
- Dynode
- Reflectron
- Inductively coupled plasma
- Ion
- Reduced inequalities
- Peace, Justice and strong institutions