Normalization of mass cytometry data with bead standards

Mass cytometry uses atomic mass spectrometry combined with isotopically pure reporter elements to currently measure as many as 40 parameters per single cell. As with any quantitative technology, there is a fundamental need for quality assurance and normalization protocols. In the case of mass cytometry, the signal variation over time due to changes in instrument performance combined with intervals between scheduled maintenance must be accounted for and then normalized. Here, samples were mixed with polystyrene beads embedded with metal lanthanides, allowing monitoring of mass cytometry instrument performance over multiple days of data acquisition. The protocol described here includes simultaneous measurements…

• DR
  Damon Runyon Cancer Research Foundation

  Award: DRG-2017-09
• UO
  University of Toronto
• NI
  National Institutes of Health

  Awards: U19 AI057229, AI057229, PN2EY018228, P01 CA034233, 1R01CA130826, HHSN272200700038C