Dynamic Biodistribution of Extracellular Vesicles in Vivo Using a Multimodal Imaging Reporter
Harvard University · Massachusetts General Hospital · +1 more institution
Abstract
Extracellular vesicles (EVs) are nanosized vesicles released by normal and diseased cells as a novel form of intercellular communication and can serve as an effective therapeutic vehicle for genes and drugs. Yet, much remains unknown about the in vivo properties of EVs such as tissue distribution, blood levels, and urine clearance, important parameters that will define their therapeutic effectiveness and potential toxicity. Here we combined Gaussia luciferase and metabolic biotinylation to create a sensitive EV reporter (EV-GlucB) for multimodal imaging in vivo, as well as monitoring of EV levels in the organs and biofluids ex vivo after administration of EVs. Bioluminescence and fluorescence-mediated…
Citation impact
- FWCI
- 15.22
- Percentile
- 100%
- References
- 40
Authors
8- CPCharles Pin‐Kuang LaiCorresponding
Harvard University, Massachusetts General Hospital, Center for Systems Biology
- OMOsama Mardini
Center for Systems Biology, Harvard University, Massachusetts General Hospital
- MEMaria Ericsson
Massachusetts General Hospital, Center for Systems Biology, Harvard University
- SPShilpa Prabhakar
Harvard University, Center for Systems Biology, Massachusetts General Hospital
- CACasey A. Maguire
Center for Systems Biology, Harvard University, Massachusetts General Hospital
Topics & keywords
- Biodistribution
- In vivo
- Ex vivo
- Bioluminescence imaging
- Extracellular vesicles
- Luciferase
- Preclinical imaging
- Distribution (mathematics)
- Good health and well-being