Induction of APOBEC3G Ubiquitination and Degradation by an HIV-1 Vif-Cul5-SCF Complex
Johns Hopkins University · Jilin University · +2 more institutions
Abstract
Human immunodeficiency virus-1 (HIV-1) Vif is essential for viral evasion of host antiviral factor CEM15/APOBEC3G. We report that Vif interacts with cellular proteins Cul5, elongins B and C, and Rbx1 to form an Skp1-cullin-F-box (SCF)-like complex. The ability of Vif to suppress antiviral activity of APOBEC3G was specifically dependent on Cul5-SCF function, allowing Vif to interact with APOBEC3G and induce its ubiquitination and degradation. A Vif mutant that interacted with APOBEC3G but not with Cul5-SCF was functionally inactive. The Cul5-SCF was also required for Vif function in distantly related simian immunodeficiency virus mac. These results indicate that the conserved Cul5-SCF pathway used by Vif is a…
Citation impact
- FWCI
- 20.01
- Percentile
- 100%
- References
- 29
Authors
7- XYXianghui YuCorresponding
Johns Hopkins University, Jilin University, Jilin Medical University, Zhejiang University
- YYYunkai YuCorresponding
Johns Hopkins University, Jilin University, Jilin Medical University, Zhejiang University
- BLBindong LiuCorresponding
Johns Hopkins University, Jilin University, Jilin Medical University, Zhejiang University
- KLKun Luo
Johns Hopkins University, Jilin University, Jilin Medical University, Zhejiang University
- WKWei Kong
Johns Hopkins University, Jilin University, Jilin Medical University, Zhejiang University
Topics & keywords
- APOBEC3G
- Cullin
- SAMHD1
- Biology
- Ubiquitin
- Cell biology
- Virology
- Infectivity
- Good health and well-being