Tissue‐specific and inducible Cre‐mediated recombination in the gut epithelium
Centre National de la Recherche Scientifique · Institut Curie · +5 more institutions
Abstract
We generated two complementary systems for Cre-mediated recombination of target genes in the mouse digestive epithelium and tested them with a Cre-reporter mouse strain. Cre was expressed under the control of a 9 kb regulatory region of the murine villin gene (vil-Cre). Genetic recombination was initiated at embryonic day (E) 9 in the visceral endoderm, and by E12.5 in the entire intestinal epithelium, but not in other tissues. Cre expression was maintained throughout adulthood. Furthermore, transgenic mice bearing a tamoxifen-dependent Cre recombinase (vil-Cre-ERT2) expressed under the control of the villin promoter were created to perform targeted spatiotemporally controlled somatic recombination. After…
Citation impact
- FWCI
- 6.60
- Percentile
- 100%
- References
- 20
Authors
10- FEFatima El Marjou
Centre National de la Recherche Scientifique, Institut Curie
- KJKlaus‐Peter Janssen
TUM Klinikum
- BHBenny Hung‐Junn Chang
Baylor College of Medicine
- MLMei Li
Centre National de la Recherche Scientifique, Inserm, Institut de génétique et de biologie moléculaire et cellulaire
- VHValérie Hindie
Centre National de la Recherche Scientifique, Institut Curie
Topics & keywords
- Villin
- Cre recombinase
- Biology
- Intestinal epithelium
- Transgene
- Gene targeting
- Molecular biology
- Epithelium