Retroviral DNA Integration: ASLV, HIV, and MLV Show Distinct Target Site Preferences

Mitchell, Rick; Beitzel, Brett; Schröder, A. R. W.; Shinn, Paul; Chen, Huaming; Berry, Charles C.; Ecker, Joseph R.; Bushman, Frederic D.

doi:10.1371/journal.pbio.0020234

articlePLoS BiologyAug 10, 2004GOLD OA

Retroviral DNA Integration: ASLV, HIV, and MLV Show Distinct Target Site Preferences

RMRick Mitchell BBBrett Beitzel ARA. R. W. Schröder PSPaul Shinn HCHuaming Chen

University of Pennsylvania · Salk Institute for Biological Studies · +1 more institution

PubMed

Indexed incrossrefdoajpubmed

Abstract

The completion of the human genome sequence has made possible genome-wide studies of retroviral DNA integration. Here we report an analysis of 3,127 integration site sequences from human cells. We compared retroviral vectors derived from human immunodeficiency virus (HIV), avian sarcoma-leukosis virus (ASLV), and murine leukemia virus (MLV). Effects of gene activity on integration targeting were assessed by transcriptional profiling of infected cells. Integration by HIV vectors, analyzed in two primary cell types and several cell lines, strongly favored active genes. An analysis of the effects of tissue-specific transcription showed that it resulted in tissue-specific integration targeting by HIV, though the…

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Topics & keywords

Topics

Keywords

Biology
Genome
Gene
Genetics
Murine leukemia virus
Virology
Computational biology

UN Sustainable Development Goals

Good health and well-being

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