Novel adeno-associated viruses from rhesus monkeys as vectors for human gene therapy
The Wistar Institute · University of Pennsylvania · +1 more institution
Abstract
Tissues from rhesus monkeys were screened by PCR for the presence of sequences homologous to known adeno-associated virus (AAV) serotypes 1-6. DNA spanning entire rep-cap ORFs from two novel AAVs, called AAV7 and AAV8, were isolated. Sequence comparisons among these and previously described AAVs revealed the greatest divergence in capsid proteins. AAV7 and AAV8 were not neutralized by heterologous antisera raised to the other serotypes. Neutralizing antibodies to AAV7 and AAV8 were rare in human serum and, when present, were low in activity. Vectors formed with capsids from AAV7 and AAV8 were generated by using rep and inverted terminal repeats (ITRs) from AAV2 and were compared with similarly constructed…
Citation impact
- FWCI
- 41.98
- Percentile
- 100%
- References
- 37
Authors
6- GGGuangping Gao
The Wistar Institute, University of Pennsylvania
- MRMauricio R. Alvira
The Wistar Institute, Princeton University, University of Pennsylvania
- LWLili Wang
The Wistar Institute, Princeton University, University of Pennsylvania
- RCRoberto Calcedo
The Wistar Institute, Princeton University, University of Pennsylvania
- JJJulie Johnston
The Wistar Institute, Princeton University, University of Pennsylvania
Topics & keywords
- Adeno-associated virus
- Biology
- Virology
- Genetic enhancement
- Capsid
- Vectors in gene therapy
- Heterologous
- Vector (molecular biology)