Replicative Senescence of Mesenchymal Stem Cells: A Continuous and Organized Process
Heidelberg University · Heidelberg Academy of Sciences and Humanities · +1 more institution
Abstract
Mesenchymal stem cells (MSC) comprise a promising tool for cellular therapy. These cells are usually culture expanded prior to their application. However, a precise molecular definition of MSC and the sequel of long-term in vitro culture are yet unknown. In this study, we have addressed the impact of replicative senescence on human MSC preparations. Within 43 to 77 days of cultivation (7 to 12 passages), MSC demonstrated morphological abnormalities, enlargement, attenuated expression of specific surface markers, and ultimately proliferation arrest. Adipogenic differentiation potential decreased whereas the propensity for osteogenic differentiation increased. mRNA expression profiling revealed a consistent…
Citation impact
- FWCI
- 24.50
- Percentile
- 100%
- References
- 61
Authors
11Topics & keywords
- Mesenchymal stem cell
- Biology
- Gene expression profiling
- Senescence
- Cell biology
- Gene expression
- Phenotype
- Stem cell