articleScienceJun 15, 2006Closed access

Characterization of the piRNA Complex from Rat Testes

Massachusetts General Hospital · Harvard–MIT Division of Health Sciences and Technology · +3 more institutions

PubMed
Indexed incrossrefpubmed

Abstract

Small noncoding RNAs regulate processes essential for cell growth and development, including mRNA degradation, translational repression, and transcriptional gene silencing (TGS). During a search for candidate mammalian factors for TGS, we purified a complex that contains small RNAs and Riwi, the rat homolog to human Piwi. The RNAs, frequently 29 to 30 nucleotides in length, are called Piwi-interacting RNAs (piRNAs), 94% of which map to 100 defined (

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