Induced Pluripotent Stem Cells Generated Without Viral Integration
Joslin Diabetes Center · Harvard University · +2 more institutions
Abstract
Pluripotent stem cells have been generated from mouse and human somatic cells by viral expression of the transcription factors Oct4, Sox2, Klf4, and c-Myc. A major limitation of this technology is the use of potentially harmful genome-integrating viruses. We generated mouse induced pluripotent stem (iPS) cells from fibroblasts and liver cells by using nonintegrating adenoviruses transiently expressing Oct4, Sox2, Klf4, and c-Myc. These adenoviral iPS (adeno-iPS) cells show DNA demethylation characteristic of reprogrammed cells, express endogenous pluripotency genes, form teratomas, and contribute to multiple tissues, including the germ line, in chimeric mice. Our results provide strong evidence that…
Citation impact
- FWCI
- 84.78
- Percentile
- 100%
- References
- 29
Authors
5- MSMatthias Stadtfeld
Joslin Diabetes Center, Harvard University, Massachusetts General Hospital, Harvard Stem Cell Institute
- MNMasaki Nagaya
Joslin Diabetes Center, Harvard University, Massachusetts General Hospital, Harvard Stem Cell Institute
- JUJochen Utikal
Joslin Diabetes Center, Harvard University, Massachusetts General Hospital, Harvard Stem Cell Institute
- GCGordon C. Weir
Joslin Diabetes Center, Harvard University, Massachusetts General Hospital, Harvard Stem Cell Institute
- KHKonrad HochedlingerCorresponding
Joslin Diabetes Center, Harvard University, Massachusetts General Hospital, Harvard Stem Cell Institute
Topics & keywords
- Induced pluripotent stem cell
- Reprogramming
- SOX2
- KLF4
- Biology
- Embryonic stem cell
- Stem cell
- Cell biology