Isolation of circulating tumor cells using a microvortex-generating herringbone-chip
Harvard University · Massachusetts General Hospital · +2 more institutions
Abstract
Rare circulating tumor cells (CTCs) present in the bloodstream of patients with cancer provide a potentially accessible source for detection, characterization, and monitoring of nonhematological cancers. We previously demonstrated the effectiveness of a microfluidic device, the CTC-Chip, in capturing these epithelial cell adhesion molecule (EpCAM)-expressing cells using antibody-coated microposts. Here, we describe a high-throughput microfluidic mixing device, the herringbone-chip, or "HB-Chip," which provides an enhanced platform for CTC isolation. The HB-Chip design applies passive mixing of blood cells through the generation of microvortices to significantly increase the number of interactions between…
Citation impact
- FWCI
- 50.48
- Percentile
- 100%
- References
- 30
Authors
23- SLShannon L. StottCorresponding
Harvard University, Massachusetts General Hospital
- CHChia‐Hsien Hsu
Harvard University, National Health Research Institutes, Massachusetts General Hospital
- DTDina Tsukrov
Harvard University, Massachusetts General Hospital
- MYMin Yu
Harvard University, Massachusetts General Hospital
- DTDavid T. Miyamoto
Harvard University, Massachusetts General Hospital
Topics & keywords
- Circulating tumor cell
- Cancer
- Antibody
- Immunofluorescence
- Epithelial cell adhesion molecule
- Cancer research
- Cancer cell
- Microfluidic chip
Funding
- UDU.S. Department of Defense
- HHHoward Hughes Medical Institute
- PCProstate Cancer Foundation
- AMAmbrose Monell Foundation
- AAstraZeneca
- MGMassachusetts General Hospital
- ASAmerican Society of Clinical Oncology
- NINational Institutes of Health
- SUStand Up To Cancer
- NCNational Cancer Institute
- DPDOD Prostate Cancer Research Program