Rapid Whole-Genome Sequencing for Investigation of a Neonatal MRSA Outbreak
University of Cambridge · Bridge University · +6 more institutions
Abstract
Isolates of methicillin-resistant Staphylococcus aureus (MRSA) belonging to a single lineage are often indistinguishable by means of current typing techniques. Whole-genome sequencing may provide improved resolution to define transmission pathways and characterize outbreaks.
We investigated a putative MRSA outbreak in a neonatal intensive care unit. By using rapid high-throughput sequencing technology with a clinically relevant turnaround time, we retrospectively sequenced the DNA from seven isolates associated with the outbreak and another seven MRSA isolates associated with carriage of MRSA or bacteremia in the same hospital.
Citation impact
- FWCI
- 48.20
- Percentile
- 100%
- References
- 27
Authors
20- CUClaudio U. KöserCorresponding
University of Cambridge, Bridge University, Health Protection Agency
- MTMatthew T. G. Holden
Wellcome Sanger Institute
- MJMatthew J. Ellington
Health Protection Agency
- EJEdward J. P. Cartwright
University of Cambridge, Bridge University, Health Protection Agency
- NMNicholas M. Brown
National Health Service, Cambridge University Hospitals NHS Foundation Trust, Health Protection Agency
Topics & keywords
- Outbreak
- Whole genome sequencing
- Biology
- Genome
- Resistome
- Multilocus sequence typing
- Typing
- Genetics