Lamins A and C but Not Lamin B1 Regulate Nuclear Mechanics
Harvard University · Brigham and Women's Hospital · +4 more institutions
Abstract
Mutations in the nuclear envelope proteins lamins A and C cause a broad variety of human diseases, including Emery-Dreifuss muscular dystrophy, dilated cardiomyopathy, and Hutchinson-Gilford progeria syndrome. Cells lacking lamins A and C have reduced nuclear stiffness and increased nuclear fragility, leading to increased cell death under mechanical strain and suggesting a potential mechanism for disease. Here, we investigated the contribution of major lamin subtypes (lamins A, C, and B1) to nuclear mechanics by analyzing nuclear shape, nuclear dynamics over time, nuclear deformations under strain, and cell viability under prolonged mechanical stimulation in cells lacking both lamins A and C, cells lacking…
Citation impact
- FWCI
- 6.00
- Percentile
- 100%
- References
- 43
Authors
7- JLJan LammerdingCorresponding
Harvard University, Brigham and Women's Hospital
- LGLoren G. Fong
University of California, Los Angeles, University of California System
- JYJulie Y. Ji
Brigham and Women's Hospital, Harvard University
- KRKaren Reue
VA Greater Los Angeles Healthcare System, University of California System, University of California, Los Angeles
- CLColin L. Stewart
Frederick National Laboratory for Cancer Research
Topics & keywords
- Lamin
- Progeria
- LMNA
- Nuclear lamina
- Biology
- Cell biology
- Cell nucleus
- Nuclear protein
- Good health and well-being