articleNature CommunicationsOct 8, 2014HYBRID OA

Combining high-throughput phenotyping and genome-wide association studies to reveal natural genetic variation in rice

Huazhong Agricultural University · Wuhan National Laboratory for Optoelectronics · +1 more institution

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Abstract

Even as the study of plant genomics rapidly develops through the use of high-throughput sequencing techniques, traditional plant phenotyping lags far behind. Here we develop a high-throughput rice phenotyping facility (HRPF) to monitor 13 traditional agronomic traits and 2 newly defined traits during the rice growth period. Using genome-wide association studies (GWAS) of the 15 traits, we identify 141 associated loci, 25 of which contain known genes such as the Green Revolution semi-dwarf gene, SD1. Based on a performance evaluation of the HRPF and GWAS results, we demonstrate that high-throughput phenotyping has the potential to replace traditional phenotyping techniques and can provide valuable gene…

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