Functional assembly of engineered myocardium by electrical stimulation of cardiac myocytes cultured on scaffolds
Harvard University · Harvard–MIT Division of Health Sciences and Technology · +1 more institution
Abstract
The major challenge of tissue engineering is directing the cells to establish the physiological structure and function of the tissue being replaced across different hierarchical scales. To engineer myocardium, biophysical regulation of the cells needs to recapitulate multiple signals present in the native heart. We hypothesized that excitation-contraction coupling, critical for the development and function of a normal heart, determines the development and function of engineered myocardium. To induce synchronous contractions of cultured cardiac constructs, we applied electrical signals designed to mimic those in the native heart. Over only 8 days in vitro, electrical field stimulation induced cell alignment and…
Citation impact
- FWCI
- 22.65
- Percentile
- 100%
- References
- 28
Authors
8- MRMilica RadisicCorresponding
Harvard University, Harvard–MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology
- HPHyoungshin Park
Harvard University, Harvard–MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology
- HSHelen Shing
Harvard University, Harvard–MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology
- TRThomas R. Consi
Harvard University, Harvard–MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology
- FJFrederick J. Schoen
Harvard University, Harvard–MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology
Topics & keywords
- Myocyte
- Stimulation
- Coupling (piping)
- Cardiac myocyte
- Tissue engineering
- Contraction (grammar)
- Cell biology
- Cardiac cell