Fatty Acid Uptake and Lipid Storage Induced by HIF-1α Contribute to Cell Growth and Survival after Hypoxia-Reoxygenation
John Radcliffe Hospital · University of Oxford · +7 more institutions
Abstract
An in vivo model of antiangiogenic therapy allowed us to identify genes upregulated by bevacizumab treatment, including Fatty Acid Binding Protein 3 (FABP3) and FABP7, both of which are involved in fatty acid uptake. In vitro, both were induced by hypoxia in a hypoxia-inducible factor-1α (HIF-1α)-dependent manner. There was a significant lipid droplet (LD) accumulation in hypoxia that was time and O2 concentration dependent. Knockdown of endogenous expression of FABP3, FABP7, or Adipophilin (an essential LD structural component) significantly impaired LD formation under hypoxia. We showed that LD accumulation is due to FABP3/7-dependent fatty acid uptake while de novo fatty acid synthesis is repressed in…
Citation impact
- FWCI
- 8.38
- Percentile
- 100%
- References
- 66
Authors
15- KBKarim BensaadCorresponding
John Radcliffe Hospital, University of Oxford, MRC Weatherall Institute of Molecular Medicine, MRC Human Immunology Unit
- EFElena Favaro
John Radcliffe Hospital, University of Oxford
- CACaroline A. Lewis
The Honourable Society of Lincoln's Inn, Cancer Research UK
- BPBarrie Peck
The Honourable Society of Lincoln's Inn, Cancer Research UK
- SLSimon Lord
John Radcliffe Hospital, University of Oxford
Topics & keywords
- Hypoxia (environmental)
- Gene knockdown
- In vivo
- Downregulation and upregulation
- Lipid metabolism
- Fatty acid
- In vitro
- Biology