reviewEuropean Journal of HistochemistryDec 12, 2014GOLD OA

Autofluorescence spectroscopy and imaging: a tool for biomedical research and diagnosis

National Academies of Sciences, Engineering, and Medicine · University of Pavia · +2 more institutions

PubMed
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Abstract

Native fluorescence, or autofluorescence (AF), consists in the emission of light in the UV-visible, near-IR spectral range when biological substrates are excited with light at suitable wavelength. This is a well-known phenomenon, and the strict relationship of many endogenous fluorophores with morphofunctional properties of the living systems, influencing their AF emission features, offers an extremely powerful resource for directly monitoring the biological substrate condition. Starting from the last century, the technological progresses in microscopy and spectrofluorometry were convoying attention of the scientific community to this phenomenon. In the future, the interest in the autofluorescence will…

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