SOX17 Is a Critical Specifier of Human Primordial Germ Cell Fate
Wellcome/MRC Cambridge Stem Cell Institute · University of Cambridge · +4 more institutions
Abstract
Specification of primordial germ cells (PGCs) marks the beginning of the totipotent state. However, without a tractable experimental model, the mechanism of human PGC (hPGC) specification remains unclear. Here, we demonstrate specification of hPGC-like cells (hPGCLCs) from germline competent pluripotent stem cells. The characteristics of hPGCLCs are consistent with the embryonic hPGCs and a germline seminoma that share a CD38 cell-surface marker, which collectively defines likely progression of the early human germline. Remarkably, SOX17 is the key regulator of hPGC-like fate, whereas BLIMP1 represses endodermal and other somatic genes during specification of hPGCLCs. Notable mechanistic differences between…
Citation impact
- FWCI
- 30.16
- Percentile
- 100%
- References
- 65
Authors
9- NINaoko Irie
Wellcome/MRC Cambridge Stem Cell Institute, University of Cambridge, The Gurdon Institute, Wellcome Trust, Medical Research Council
- LWLeehee Weinberger
Weizmann Institute of Science
- WWWalfred W. C. Tang
Wellcome/MRC Cambridge Stem Cell Institute, University of Cambridge, The Gurdon Institute, Wellcome Trust, Medical Research Council
- TKToshihiro Kobayashi
Wellcome/MRC Cambridge Stem Cell Institute, University of Cambridge, The Gurdon Institute, Wellcome Trust, Medical Research Council
- SVSergey Viukov
Weizmann Institute of Science
Topics & keywords
- Biology
- Germline
- Somatic cell
- Epigenome
- Reprogramming
- Induced pluripotent stem cell
- Embryonic stem cell
- Genetics