Macrophage Polarisation: an Immunohistochemical Approach for Identifying M1 and M2 Macrophages
Unfallkrankenhaus Berlin · Sana Klinikum Lichtenberg · +1 more institution
Abstract
Macrophage polarization is increasingly recognised as an important pathogenetic factor in inflammatory and neoplastic diseases. Proinflammatory M1 macrophages promote T helper (Th) 1 responses and show tumoricidal activity. M2 macrophages contribute to tissue repair and promote Th2 responses. CD68 and CD163 are used to identify macrophages in tissue sections. However, characterisation of polarised macrophages in situ has remained difficult. Macrophage polarisation is regulated by transcription factors, pSTAT1 and RBP-J for M1, and CMAF for M2. We reasoned that double-labelling immunohistochemistry for the detection of macrophage markers together with transcription factors may be suitable to characterise…
Citation impact
- FWCI
- 10.45
- Percentile
- 100%
- References
- 45
Authors
5Topics & keywords
- Macrophage
- Immunohistochemistry
- Biology
- Pathology
- Medicine
- Immunology
- Genetics
- Good health and well-being