Widespread occurrence of 5-methylcytosine in human coding and non-coding RNA
Victor Chang Cardiac Research Institute · UNSW Sydney · +2 more institutions
Abstract
The modified base 5-methylcytosine (m(5)C) is well studied in DNA, but investigations of its prevalence in cellular RNA have been largely confined to tRNA and rRNA. In animals, the two m(5)C methyltransferases NSUN2 and TRDMT1 are known to modify specific tRNAs and have roles in the control of cell growth and differentiation. To map modified cytosine sites across a human transcriptome, we coupled bisulfite conversion of cellular RNA with next-generation sequencing. We confirmed 21 of the 28 previously known m(5)C sites in human tRNAs and identified 234 novel tRNA candidate sites, mostly in anticipated structural positions. Surprisingly, we discovered 10,275 sites in mRNAs and other non-coding RNAs. We observed…
Citation impact
- FWCI
- 15.72
- Percentile
- 100%
- References
- 58
Authors
8- JEJeffrey E. SquiresCorresponding
Victor Chang Cardiac Research Institute, UNSW Sydney, St Vincent's Clinic, Australian National University
- HRHardip R. Patel
Victor Chang Cardiac Research Institute, St Vincent's Clinic, Australian National University, UNSW Sydney
- MNMarco Nousch
St Vincent's Clinic, Victor Chang Cardiac Research Institute, UNSW Sydney, Australian National University
- TSTennille Sibbritt
Victor Chang Cardiac Research Institute, UNSW Sydney, Australian National University, St Vincent's Clinic
- DTDavid T. Humphreys
Australian National University, St Vincent's Clinic, UNSW Sydney, Victor Chang Cardiac Research Institute
Topics & keywords
- Biology
- RNA
- Transfer RNA
- Non-coding RNA
- Genetics
- RNase P
- Transcriptome
- Small nucleolar RNA