articleBloodJan 10, 2008BRONZE OA

MicroRNA signatures associated with cytogenetics and prognosis in acute myeloid leukemia

The Ohio State University · University of Ferrara · +6 more institutions

PubMed
Indexed incrossrefpubmed

Abstract

MicroRNAs (miRNAs) are small RNAs of 19 to 25 nucleotides that are negative regulators of gene expression. To determine whether miRNAs are associated with cytogenetic abnormalities and clinical features in acute myeloid leukemia (AML), we evaluated the miRNA expression of CD34(+) cells and 122 untreated adult AML cases using a microarray platform. After background subtraction and normalization using a set of housekeeping genes, data were analyzed using Significance Analysis of Microarrays. An independent set of 60 untreated AML patients was used to validate the outcome signatures using real-time polymerase chain reaction. We identified several miRNAs differentially expressed between CD34(+) normal cells and…

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Authors

18

Topics & keywords

Keywords
  • Cytogenetics
  • Myeloid leukemia
  • microRNA
  • Medicine
  • Leukemia
  • Myeloid
  • Biology
  • Cancer research
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