Relating the metatranscriptome and metagenome of the human gut

Although the composition of the human microbiome is now well-studied, the microbiota's >8 million genes and their regulation remain largely uncharacterized. This knowledge gap is in part because of the difficulty of acquiring large numbers of samples amenable to functional studies of the microbiota. We conducted what is, to our knowledge, one of the first human microbiome studies in a well-phenotyped prospective cohort incorporating taxonomic, metagenomic, and metatranscriptomic profiling at multiple body sites using self-collected samples. Stool and saliva were provided by eight healthy subjects, with the former preserved by three different methods (freezing, ethanol, and RNAlater) to validate…

• NS
  National Science Foundation

  Awards: 1053486, DBI-1053486
• DR
  Damon Runyon Cancer Research Foundation
• D
  Danone

  Award: PLF-5972-GD
• AG
  American Gastroenterological Association

  Award: R01 CA137178
• BI
  Broad Institute
• NI
  National Institutes of Health

  Awards: DK043351, DBI-1053486, P30 DK043351, R01 CA137178, HHSN272200900018C, P50 CA127003, NIH R01
• NI
  National Institute of Allergy and Infectious Diseases

  Award: HHSN272200900018C
• AR
  Army Research Office

  Awards: W911NF-11-1-0473, W911NF, W911NF-11