Nrf2 regulates ROS production by mitochondria and NADPH oxidase
University College London · National Hospital for Neurology and Neurosurgery · +5 more institutions
Abstract
Nuclear factor (erythroid-derived 2) factor 2 (Nrf2) is a crucial transcription factor mediating protection against oxidants. Nrf2 is negatively regulated by cytoplasmic Kelch-like ECH associated protein 1 (Keap1) thereby providing inducible antioxidant defence. Antioxidant properties of Nrf2 are thought to be mainly exerted by stimulating transcription of antioxidant proteins, whereas its effects on ROS production within the cell are uncertain.
Live cell imaging and qPCR in brain hippocampal glio-neuronal cultures and explants slice cultures with graded expression of Nrf2, i.e. Nrf2-knockout (Nrf2-KO), wild-type (WT), and Keap1-knockdown (Keap1-KD).
Citation impact
- FWCI
- 13.37
- Percentile
- 100%
- References
- 65
Authors
6- SKStjepana Kovac
University College London, National Hospital for Neurology and Neurosurgery, University of Münster
- PRPlamena R. Angelova
National Hospital for Neurology and Neurosurgery, University College London
- KMKira M. Holmström
National Hospital for Neurology and Neurosurgery, University College London
- YZYing Zhang
Ninewells Hospital, University of Dundee
- ATAlbena T. Dinkova‐KostovaCorresponding
Johns Hopkins University, Johns Hopkins Medicine
Topics & keywords
- KEAP1
- Cell biology
- NADPH oxidase
- NOX4
- Transcription factor
- Mitochondrial ROS
- Mitochondrion
- Gene knockdown