Regulation of Cytokine Receptors by Golgi N-Glycan Processing and Endocytosis
Mount Sinai Hospital · University of British Columbia · +3 more institutions
Abstract
The Golgi enzyme beta1,6 N-acetylglucosaminyltransferase V (Mgat5) is up-regulated in carcinomas and promotes the substitution of N-glycan with poly N-acetyllactosamine, the preferred ligand for galectin-3 (Gal-3). Here, we report that expression of Mgat5 sensitized mouse cells to multiple cytokines. Gal-3 cross-linked Mgat5-modified N-glycans on epidermal growth factor and transforming growth factor-beta receptors at the cell surface and delayed their removal by constitutive endocytosis. Mgat5 expression in mammary carcinoma was rate limiting for cytokine signaling and consequently for epithelial-mesenchymal transition, cell motility, and tumor metastasis. Mgat5 also promoted cytokine-mediated leukocyte…
Citation impact
- FWCI
- 7.76
- Percentile
- 100%
- References
- 27
Authors
9- EAEmily A. Partridge
Mount Sinai Hospital, University of British Columbia, University of Toronto, Lunenfeld-Tanenbaum Research Institute, Institute of Cell Biology and Neurobiology
- CLChristine Le Roy
Mount Sinai Hospital, University of British Columbia, University of Toronto, Lunenfeld-Tanenbaum Research Institute, Institute of Cell Biology and Neurobiology
- GMGianni M. Di Guglielmo
Mount Sinai Hospital, University of British Columbia, University of Toronto, Lunenfeld-Tanenbaum Research Institute, Institute of Cell Biology and Neurobiology
- JPJudy Pawling
Mount Sinai Hospital, University of British Columbia, University of Toronto, Lunenfeld-Tanenbaum Research Institute, Institute of Cell Biology and Neurobiology
- PCPam Cheung
Mount Sinai Hospital, University of British Columbia, University of Toronto, Lunenfeld-Tanenbaum Research Institute, Institute of Cell Biology and Neurobiology
Topics & keywords
- Endocytosis
- Cell biology
- Cytokine
- Receptor
- Biology
- Glycan
- Galectin
- Golgi apparatus
- Good health and well-being